HyCyte Cell Line: HyCyte Huh-7 KO-hSLC39A1

Parental: Huh-7

Catalogue No.: CGKO-M2476

Species: Human

Gene: hSLC39A1

Gene ID: 27173

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 2mM Glutamax,100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G217).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hSLC39A1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative