HyCyte Cell Line: HyCyte MDA-MB-231 KO-hHNRNPA2B1

Parental: MDA-MB-231

Catalogue No.: CGKO-M2470

Species: Human

Gene: hHNRNPA2B1

Gene ID: 3181

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Leibovitz L-15 Medium supplemented with 10 % FBS, 100 U/mL Penicillin and 100 µg/mL Streptomycin (HyCyte,TCH-G248).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hHNRNPA2B1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative