HyCyte Cell Line: HyCyte 22Rv1 KO-hPTEN

Parental: 22Rv1

Catalogue No.: CGKO-M2454

Gene: hPTEN

Gene ID: 5728

Passage: 9

Doubling time: 24-48 h

Clone Type: Homozygote

Recommended culture media:

RPMI-1640   supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G100).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO; or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hPTEN (-/-)

Quality test: Bacteria, fungi and mycoplasma negative