HyCyte Cell Line: HyCyte DLD-1 KO-hBRCA1

Parental: DLD-1

Catalogue No.: CGKO-M2435

Species: Human

Gene: hBRCA1

Gene ID: 672

Passage: 9

Doubling time: 48-72 h

Growth Properties：Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640   supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G169).

Subculture:

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every

2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO; or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hBRCA1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative