HyCyte Cell Line: HyCyte HCC-LM3 KO-hHSPA4

Parental: HCC-LM3

Catalogue No.: CGKO-M2406

Gene: hHSPA4

Gene ID: 3308

Passage: 9

Doubling time: 36-48 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G456).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hHSPA4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative