HyCyte Cell Line: HyCyte U-87MG KO-hTP53

Parental: U-87MG

Catalogue No.: CGKO-M2376

Species: Human

Gene: hTP53

Gene ID: 7157

Passage: 9

Doubling time: 36-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G367).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hTP53 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative