HyCyte Cell Line: HyCyte A-498 KO-hCBX7

Parental:   A-498

Catalogue No.: CGKO-M2370

Species: Human

Gene: hCBX7

Gene ID: 23492

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G451).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hCBX7 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative