HyCyte Cell Line: HyCyte LM8 KO-mFgl2

Parental: LM8

Catalogue No.: CGKO-M2362

Species: Mouse

Gene: mFgl2

Gene ID: 14190

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12)   supplemented with 10 % FBS,, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G814).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO; or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mFgl2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative