HyCyte Cell Line: HyCyte Caco-2 KO-hAPOA1

Parental: Caco-2

Catalogue No.: CGKO-M2347

Gene: hAPOA1

Gene ID: 335

Passage: 9

Doubling time: 60-80 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 20 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G146).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hAPOA1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative