HyCyte Cell Line: HyCyte MAC-T KO-cwSIRT5

Parental: MAC-T

Catalogue No.: CGKO-M2323

Gene: cwSIRT5

Gene ID: 507347

Passage: 9

Doubling time: 24-36 h

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCO-G040).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: cwSIRT5 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative