HyCyte Cell Line: HyCyte NCI-H520 KO-hPTPRZ1

Parental: NCI-H520

Catalogue No.: CGKO-M2278

Gene: hPTPRZ1

Gene ID: 5803

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G427).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hPTPRZ1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative