HyCyte Cell Line: HyCyte HEK293T KO-hERAP1 & hB2M

Parental: HEK293T

Catalogue No.: CGKO-M2214

Species: Human

Gene: hERAP1 & hB2M

Gene ID: 51752 & 567

Passage: 9

Doubling time: 24-48 h

Clone Type: Double knockout homozygous

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G101).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hNQO1&hNQO2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative