HyCyte Cell Line: HyCyte HTR-8/SVneo KO-hADGRA2

Parental: HTR-8/SVneo

Catalogue No.: CGKO-M2212

Gene: hADGRA2

Gene ID: 25960

Passage: 9

Doubling time: 24-48 h

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 5 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G385).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:5.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hADGRA2 (-/-)

Quality test: bacteria, fungi and mycoplasma negative