HyCyte Cell Line: HyCyte MeT-5A KO-hBAP1

Parental: MeT-5A

Catalogue No.: CGKO-M2211

Species: Human

Gene: hBAP1

Gene ID: 8314

Passage: 9

Doubling time: 24-36 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 3.3 nM EGF, 400 nM hydrocortisone, 10 ug/mL Human recombinant insulin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G386).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hBAP1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative