HyCyte Cell Line: HyCyte K-562 KO-hERAP1

Parental: K-562

Catalogue No.: CGKO-M2197

Species: Human

Gene: hERAP1

Gene ID: 51752

Passage: 9

Doubling time: 24-48 h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

Iscove’s Modified Dulbecco Medium(IMDM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL

Streptomycin (HyCyte,TCH-G226).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: IMDM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hERAP1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative