HyCyte Cell Line: HyCyte THP-1 KO-TNFR1 & TNFR2

Parental: THP-1

Catalogue No.: CGKO-M2155

Species: Human

Gene: TNFR1 & TNFR2

Gene ID: —

Passage: 9

Doubling time: 24-48 h

Growth Properties: Suspension

Clone Type: Double knockout homozygous

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G361).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: TNFR1 & TNFR2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative