HyCyte Cell Line: HyCyte MDA-MB-468 KO-hRALA

Parental: MDA-MB-468

Catalogue No.: CGKO-M2191

Species: Human

Gene: hRALA

Gene ID: 5898

Passage: 9

Doubling time: 72-96 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Leibovitz L-15 Medium   supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G253).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 0% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hRALA (-/-)

Quality test: Bacteria, fungi and mycoplasma negative