HyCyte Cell Line: HyCyte C1498-Fluc-Puro KO-mTrem2

Parental: C1498-Fluc-Puro

Catalogue No.: CGKO-M2184

Gene: mTrem2

Gene ID: 83433

Passage: 9

Doubling time: 24-48 h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 1.0 ug/mL Puromycin,100 U/mL Penicilin and 100 µg/mL

Streptomycin (HyCyte,TCM-G785L).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mTrem2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative