HyCyte Cell Line: HyCyte HCT 15 KO-hBRCA2

Parental: HCT 15

Catalogue No.: CGKO-M2178

Species: Human

Gene: hBRCA2

Gene ID: 675

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL

Streptomycin (HyCyte,TCH-G186).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hBRCA2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative