HyCyte Cell Line: HyCyte BT-549 KO-hPDK1

Parental: BT-549

Catalogue No.: CGKO-M2168

Species: Human

Gene: hPDK1

Gene ID: 5163

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 10 ug/mL Human recombinant insulin,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G140).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:5.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hPDK1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative