HyCyte Cell Line: HyCyte NK-92 KO-ADORA2A

Parental: NK-92

Catalogue No.: CGKO-M2158

Species: Human

Gene: ADORA2A

Gene ID: /

Passage: 9

Doubling time: 48-72 h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium α(MEM α)supplemented with 12.5 % FBS, 12.5 % HS, 0.2mM Inositol, 0.05 mM β-mercaptoethanol, 0.02mM Folic Acid, 100 U/ml Recombinant IL-2, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G292).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: MEMα + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: ADORA2A (-/-)

Quality test: Bacteria, fungi and mycoplasma negative