HyCyte Cell Line: HyCyte Hep 3B2.1-7 KO-hKLF4

Parental: Hep 3B2.1-7

Catalogue No.: CGKO-M2124

Species: Human

Gene: hKLF4

Gene ID: —

Passage: 9

Doubling time: 72-96 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,1% NEAA,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G195).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hKLF4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative