HyCyte Cell Line: HyCyte NCI-H1299 KO-hUBE3A

Parental: NCI-H1299

Catalogue No.: CGKO-M0007

Gene: hUBE3A

Gene ID: 7337

Passage: 9

Doubling time: 48-72 h

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G269).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hUBE3A (-/-)

Quality test: Bacteria, fungi and mycoplasma negative