HyCyte Cell Line: HyCyte Ramos KO-hSH3KBP1

Parental: Ramos

Catalogue No.: CGKO-M0001

Species: Human

Gene: hSH3KBP1

Gene ID: 30011

Passage: 9

Doubling time: 48-72 h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G390).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hSH3KBP1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative